Prometheus

Prometheus Panta

The new gold standard for precise characterization of protein stability

Overview

The Prometheus Panta system offers precise, label-free biophysical characterization for proteins, enabling rapid and easy determination of comprehensive stability profiles for molecules such as antibodies, ADCs, and enzymes. Its exceptional versatility makes it an ideal tool for a wide range of applications, from discovery and preclinical drug development to academic research, providing reliable insights across various stages of the research process.

For high-throughput applications, Panta can be configured with a Robotic Autosampler (robotic arm, chassis, control software). This enables fully automated, hands-free analyses on up to 1536 samples.

High resolution and sensitivity

Detect multiple unfolding events or distinguish differences in unfolding profiles even among proteins with similar T_ms even at low sample concentrations.

Precision

Provide replicate measurements with minimal deviation between individual samples.

Broad dynamic range

Measure samples at high or low concentrations.

High level of specificity

Distinguish the signal of your protein of interest from the signal of the buffer or matrix, without the need for a blank or calibration.

Multi-parameter with minimal sample

Measure multiple parameters in less than two hours, using only 10 μL sample.

The Prometheus Panta system combines four advanced biophysical characterization technologies: nanoDSF, DLS, SLS, and Backreflection. Capable of both isothermal measurements and data acquisition across an entire thermal ramp, this system offers unmatched versatility.

NanoDSF

Measures thermal unfolding or chemical denaturation under native conditions and label-free by detecting changes in its intrinsic fluorescence during a thermal ramp or in the presence of a chemical denaturant

Measurement parameters: ratio: T_onset, T_m, ∆∆G, ∆G, C₅₀, E_a, isothermal stability, reversibility of unfolding, similarity, %-folded

DLS

Detects molecule aggregates to determine the size of proteins over a wide concentration range

Measurement parameters: T_scattering, T_size, r_H, PDI, k_D, B_22,D₀

SLS

Look at the overall average scattering intensity of your solution and correlates that to the molecular weight of your particles

Measurement parameters: ratio: T_onset, T_m, ∆∆G, ∆G, C₅₀, E_a, isothermal stability, reversibility of unfolding, similarity, %-folded

Backreflection

Determines aggregation status by measuring light intensity loss due to scattering

Measurement parameters: T_turbidity, reversibility of unfolding

For monitoring thermal stability during biologics formulation, developability, production, and manufacturing; evaluating target-ligand complexes before affinity-based screening; or optimizing conditions for structural biology conditions

For screening recombinant proteins expressed from different constructs, biologics formulation screening, sample optimization for biophysical assays, and characterization of self-interactions

For determining if complexes have formed, measuring how much genetic material a viral vector contains, or extrapolating how proteins will interact with themselves at high concentrations.

To indicate the presence of large aggregates by measuring light intensity loss due to scattering, i.e., turbidity