PEPperCHIP® Custom Peptide Microarrays
Specifications
From single antigens translated into overlapping peptides to whole virus proteomes – PEPperCHIP® Custom Peptide Microarrays can be fully tailored in a uniquely cost-effective manner.
  • Peptide Synthesis: On-chip using Fmoc chemistry and routine double coupling steps
  • Microarray Format: Standard slide format (3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm)
  • Number of Peptides: From 130 to 5,500 peptides printed in duplicate
  • Arrays Copies per Chip: From single arrays to 16 arrays per chip depending on the number of peptides
  • Array Content: Custom protein-derived overlapping peptides, peptide collections, substitution scans etc.
  • Available Amino Acids: L- and D-amino acids, citrulline and other amino acids on request
  • Peptide Length: Routine synthesis of up to 15mer peptides, longer peptides on request
  • Control Peptides: HA (YPYDVPDYAG) and polio (KEVPALTAVETGAT) epitopes, other controls on request
Input Data & Sample Material
  • 10-20 µl serum/plasma or 10-20 µg purified antibody
  • Protein sequences (e.g. FASTA format or UniProt ID) or peptide collections (Excel, Word or text files
Analysis
PEPperCHIP® Peptide Microarray analysis is straightforward and can be done in your own lab or by PEPperPRINT experts within a PEPperMAP® Service Offer. Further information about lab requirements for peptide microarray analysis can be found here.
Timeline
From peptide array design to production and quality control, it takes about 3 weeks.

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