Microscoop® Mint
Microscopy-guided protein isolation and identification
Overview
Syncell’s Microscoop® Mint is a groundbreaking spatial proteomics platform that has been used to reveal novel protein constituents at specific subcellular regions of interest for many biological problems.
Microscoop® labels and analyzes all proteins in a specific region of interest without pre-selection, allowing mass spec to capture low-abundance or rare proteoforms, and reveal otherwise unknown protein constituents with higher dynamic range and specificity than laser microdissection or proximity labeling methods.
It is an unprecedented spatial pulldown technology that enables unbiased subcellular proteomic identification in high resolution, high sensitivity, and high specificity.
Unbiased discovery
Extract all proteomic information without antibodies or targeted panels across an entire sample.
Broad sample compatibility
Achieve unbiased high-precision proteomic discovery of FFPE/fresh frozen tissue samples or fixed cell culture samples.
Superior sensitivity
Discover low copy number proteins from increased dynamic range due to subcellular protein isolation.
High resolution
350 nm precision at sub-cellular scale
Unmatched specificity
Two photon guided photo-biotinylation identifies only proteins in regions of interest.
Identify targets faster
Discover novel biomarkers or therapeutic targets of disease-associated locations.
Reveal
Novel proteomes of organelles, cells and organisms.
How Microscoop® Works
Step 1 - Real-time Image Analysis
Photolabeling kit (i.e. Synlight-Rich™ Kit) is first added to cells (Fixed, Frozen) or tissue (FFPE, Fresh Frozen, PFA/Methanol fixed) samples for a photochemical reaction. Microscoop® takes an image (or images of multiple colours) of the sample at one field of view (FOV) at a time. The image or images are analyzed in real time by traditional image processing or AI deep learning to segment the user's region of interest.
Step 3 - Protein Extraction
Photolabeled cells or tissues are scraped and lysed using protein extraction kit (SynPull™ Kit), and biotinylated proteins are extracted from the samples to achieve spatial protein purification. Materials from multiple slides or chambers can be pooled together to increase total protein contents.
Step 2 - Patterned Photo-Biotinylation
A femtosecond light source is controlled to illuminate the segmented region of interest one paime. This pattern illumination triggers targeted protein photo-biotinlyation in high spatial precision through the reactions of light-sensitive proves Synlight-Rich™ Kit. This patterned photolabeling is repeated the thousands of FOVs automatically to ensure that enough proteins are biotinylated for later proteomics analysis using mass spectrometry.
Step 4 - Proteomic Identification and Analysis
The collected peptides are sent to a mass spectrometer to perform LC-MS/MS analysis. Proteomes of both the photo-labeled and unlabeled samples are obtained. By comparing the control and photolabeled proteomes, a location-specific proteome at the region of interest is obtained in high sensitivity, high specificity and high spatial precision.
Reveal novel protein constituentsUnbiased discovery of new protein constituents at your region of interest (ROI)
Spatial Proteomics at Subcellular ResolutionEnables precise subcellular collection of protein complexes, offering true spatial resolution
High Sensitivity and SpecificityCaptures low-abundance protein,enabling the discovery of novel binding partners and dynamic protein networks.
Sample accessWork with FFPE/fresh frozen tissue or fixed, frozen cells
