Icon16™ and Icon96™
World’s First Real-time Thermocycler with individually controlled wells
Overview
IconPCR™ powers the next generation of library preparation with intelligent, real-time amplification. Featuring AutoNorm technology, it automatically normalises every sample during PCR — delivering sequencing-ready libraries in a single streamlined workflow, without cleanup steps or additional reagents. Available on both 16 reaction conditions (Icon16™) and 96 reaction conditions (Icon96™), it ensures consistent, high-quality data from every sample.
Independently programmable thermal zones
Each well is a fully isolated thermal zone, allowing exact protocol matching per sample—regardless of input amount or assay type.
Real-Time Feedback Per Well
Real-time fluorescence detection in each independently controlled well enables live monitoring of amplification curves. This feedback powers in-run decision-making, not just post-run analysis.
AutoNorm™
IconPCR dynamically adjusts cycle numbers per well to normalize output concentrations across all samples—without manual input, added reagents, or downstream correction steps
iconPCR Workflow:
- Prepare pre-PCR reaction mix using your standard reagents plus an intercalator of your choice
- Load samples into a standard 96-well optical plate and seal
- Select a protocol and start the run
- IconPCR monitors amplification in real time and automatically adjusts cycles per well
- Remove plate—libraries are normalized and ready to pool or proceed to cleanup
Amplify. Quantify. Normalize. All at Once
Independent well controlOptimal cycling per sample
Real-time per-well feedbackDynamically adjusts cycles during the run
No Proprietary consumablesCompatible with your existing reagents and workflows
Built-in normalizationEliminates pre- and post-PCR quantification and normalization steps
Sequencing-ready output
No individual SPRI cleanupsOne cleanup on the final pool
