Dianthus

For your challenging affinity screening

Overview

Dianthus is a cutting-edge, plate-based affinity screening platform that stands out with its microfluidics-free design, offering mass-independent measurements in solution. This innovative approach makes it the perfect solution for challenging screening campaigns. Traditional techniques like immobilization-dependent SPR often fall short when it comes to complex applications such as PROTAC binary and ternary complexes, fragment libraries, and intrinsically disordered proteins. Dianthus, however, excels in these areas, providing the sensitivity and precision needed to tackle even the most demanding drug discovery challenges.

Measure the broadest range of affinities

Dianthus detects a wide range of binding affinities — picomolar to millimolar.

Immobilization-free measurement

Analyzing interactions in close-to-native conditions, especially when dealing with challenging targets.

Consume small amounts of target and compounds

Saving on costly samples and library compounds means you can do more screening campaigns or projects.

Trusted screening platform

From hit identification to lead validation and optimization all in one platform.

Ideal for challenging molecules

Fragment libraries

Dianthus overcomes both issues with mass-independent measurements and an ability to detect a broad range of affinities.

PROTACs and other protein degraders

With Dianthus, measurements are done in solution and are mass-independent — just what your PROTAC screening project needs.

Intrinsically disordered proteins (IDPs)

Dianthus uses low target concentrations, aggregation of the IDPs won’t get in the way of screening for binders.

By integrating Spectral Shift Technologies and TRIC for measuring molecular interactions, the combined biophysical techniques provide superior sensitivity, ensuring the detection of more binders and reducing false negatives. You can confidently work with real-life samples without concerns about aggregates, impurities, or precipitates.

Spectra Shift

To measure affinity

Dianthus is the first affinity screening platform to use Spectral Shift. Label one binding partner with a fluorophore, then mix a fixed amount of it with a dilution series of the ligand. After excitation at 590nm of the mix, the binding events are detected as shifts of spectra towards either blue or red. The detection of spectral shifts is achieved through dual-wavelength detection at exactly 650nm and 670nm in an isothermal environment — this makes Dianthus so precise and able to detect very subtle shifts. Then the Kd is calculated by plotting the ratios of the fluorescence intensities from the two wavelengths against the ligand concentration.

TRIC

To quantify molecular interactions

labeling your target molecule with a fluorescent dye and mixing it with your ligand. Then, a very precise and brief laser-induced temperature change is applied, which amplifies the variation in fluorescence intensity caused by the ligand binding to your target. The change in fluorescence is measured and plotted against the ligand concentration to obtain the dissociation constant or Kd.