CEinfinite multifunction iCIEF platform
CEInfinite iCIEF instrument systems deliver high-throughput precision while being incredibly robust. The CEInfinite can flexibly switch between online iCIEF-MS and offline preparative iCIEF.
iCIEF-MS online coupling mode
• High throughput, fast method development.
• Directly couple with major brands of MS.
• Unique coatings and chemicals to accelerate the drug development process.
• Provides significant information about the quality of the drug substance within an hour.
Offline preparative mode
• Enables in-depth analysis of modifications such as deamidation at the peptide map level.
• Preparation sensitivity <0.1 pI difference. Typical automated one run is <60 min.
• A fully automated system allows Up to 96 fractions per run,~100 μg collections per day under temperature controlled environment.
• Achieve as high as 90% purity. Get as high as 8 μg isomer per run.
Capillary Diameter Transfer Technology
Our proprietary Capillary Diameter Transfer Technology (CDTT*) allows the high-resolution preparation of charge variant peaks. Once the proteins are focused, the mobilization process begins and the protein isomers are pushed out of transfer capillary. This is visualized in real time on-screen via the Whole Column Image Detection (WCID) of icIEF, which is not presented in the single-point detection cIEF.
During mobilization, the electric field is still applied to the separation capillary and the CDTT maintains the separation resolution of the sample into the narrower transfer capillary for high purity fractions of low pI different isomers.
Superior Preparative PerformanceResolution > 0.1 pI, typical automated one run is <1 hour.
High Protein Fractionation Efficiency>90% purity. Get as high as 8 µg isomer per run
Fast method integration and adoptionMinimum requirement of additional R&D derived from existing icIEF platform method
High Reproducibility and EfficiencyA fully automated system allows ~100 µg collections per day
