Andromeda X

Monitor protein expression

Overview

Andromeda X harnesses the power of thermal unfolding to optimize expression conditions and enhance protein stability. Effortlessly screen for optimal expression levels, thermal stability, and ligand binding—all in one streamlined workflow. Gain valuable insights into your recombinant proteins and accelerate your research with confidence.

Flexible Sample and Buffer Compatibility

Capable of analyzing any protein size in any buffer, including complex biological samples.

Minimal Sample Volume

Supports 48 parallel measurements with just 10 µl samples each, enabling direct loading from containers without pipetting and accommodating viscous samples up to 50 mPa s-1.

Minimal Sample Preparation

No need for blank/reference samples, filtration, dialysis, or pipetting.

High Throughput

Processes up to 48 samples per run with a daily maximum of 3000 replicates, utilizing the fastest heating rate.

Rapid, Continuous Data Acquisition

Continuous sample scanning at specific wavelength windows of 650 nm and 670 nm provides dense, high-quality data at 20 points per minute, facilitating the high scanning rate.

Highly sensitive Spectral Shift optics selectively detect your protein of interest in lysate

Spectral Shift

Mix a Nanotemper RED fluorescent label with your collected lysate containing the protein of interest. The labeling kits offer a variety of options, including His-tag and Biotinylated site-selective strategies. Once your protein is labeled, it is subjected to a thermal ramp, during which the fluorescent properties are monitored to detect protein unfolding and expression levels.