SIMPLE WESTERN SYSTEMS – WES – GEL FREE, BLOT FREE, HANDS FREE

SIMPLE WESTERN SYSTEMS – WES – GEL FREE, BLOT FREE, HANDS FREE

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Wes is an Automated, Quantitative Western analysis system that performs all the steps in a traditional western, automatically for you; from aspirating reagents and separation of proteins, immobilisation of proteins, blocking, washing, immunoprobing and ECL detection/imaging. Your results are presented to you as an electropherogram and virtual gel image and tabulated results for molecular weights, peak areas and concentrations.

  • more SENSITIVE than most traditional westerns, LOD ~1pg

  • FAST - 3hr maximum run-time with 25 samples, and only 15-30 mins of hands on/prep time in total

  • 12- 440kDa sizing range

  • Truly QUANTITATIVE as transfer step has been removed and replaced with immobilising the separated proteins to the capillary surface

  • Total Protein Assay available – replaces coomassie gels

  • Uses ~5x less protein in samples -> Low starting sample volume, 3-5ul, 0.2-0.8mg/ml needed of protein/sample

  • Uses lower amounts of precious antibodies

  • Optimisation in 1-2 runs instead of weeks, multiplexing possible

  • REPRODUCIBLE - as the critical liquid handling/loading is handled by the system

  • Higher throughput systems and charge assay functionality are available with the Sally Sue and Peggy Sue platforms

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