NanoTemper Monolith – Microscale Thermophoresis Binding Affinity (MST)

MicroScale Thermophoresis determines binding affinities in solution. It measures the motion of molecules along microscopic temperature gradients and detects changes in their hydration shell, charge or size.

Time and cost effectiveness

  • fast measurement: Kd in 10 min
  • low sample consumption: minimal concentration (pM/nM) and small volume (< 4 µl)
  • intuitive software
  • small instrument footprint, maintenance free

Easy sample preparation

  • immobilization free, in-solution measurements
  • truly label-free (optional)
  • excellent selectivity using a fluorescent dye or fluorescent protein

Broad application range

  • access affinities (Kd, dissociation constant): pM/nM to mM range
  • examine: proteins, peptides, ribosomes, ions, nucleosomes, liposomes and more
  • study membrane proteins directly in liposomes or detergent solution
  • study multi-component reactions such as ternary complex formation, order of assembly, interfering factors, cooperativity
  • measure in any buffer, including complex detergent mixtures
  • study stoichiometry and discriminate between different binding sites on a target
  • study the binding energetics ΔG (free energy), ΔH (enthalpy) and ΔS (entropy)

NanoTemper Prometheus – NanoDSF Protein Stability

nanoDSF is an advanced Differential Scanning Fluorimetry method for measuring ultra-high resolution protein stability and protein aggregation in a truly label-free approach. Applications include antibody engineering, membrane protein research, formulation and quality control.

Key Features:

  • native DSF: no dye is required
  • tyrosine and tryptophan fluorescence
  • dual UV system: 330 nm and 350 nm fluorescence is detected 48 samples at a time
  • ultra-high resolution: measure 48 capillaries in 7 seconds and see more unfolding transitions
  • broad concentration range: from 5 µg/ml to more than 250 mg/ml
  • temperature range: from 15 °C to 95 °C
  • thermal and chemical denaturation
  • maintenance-free instrument
  • straightforward handling: simple sample preparation and intuitive software user interface

Key Features of the Backreflection Optics:

  • optional backreflection optics: aggregation of protein samples is recorded
  • ultra-high resolution: precisely determine aggregation onset temperatures
  • broad concentration range: from 100 µg/ml to more than 100 mg/ml

NanoTemper Seismos – Surface Acoustic Wave Conformational Changes

Surface Acoustic Wave (SAW) is a biosensor method that uses high-frequency waves to measure conformational changes and binding kinetics.

Key features:

  • binding-induced conformational changes
  • binding kinetics: kon, koff, Kd
  • on-line sample loading
  • highest fluidic throughput: 8 sensors on two sensor chips
  • optimized for industrial applications