Mutation Discovery

Mutations in PCR products are detected by the presence of heteroduplexes optimally detected by our LCGreen® Plus dye. Variations are identified by changes in the shape of the melting profile compared to a reference sample. Hi-Res Melting™ on the LightScanner is also being used for mutation discovery in tumor samples, yielding information on mutations present in low allele fractions (as low as 5%) and allelic imbalances between tumor and matched normal samples.

Genotyping

Genotyping is possible with Hi-Res Melting eliminating the need for labeled probes. It is achieved by Hi-Res Melting™ in two ways:

1. With unlabeled 3' blocked probes data from the probe and PCR product can be obtained simultaneously.
2. Small amplicons often as short as 50 ~ 60 bp with primers just flanking the SNP. As this application relies on subtle differences in melting temperature, it is best performed on our instruments which have the best temperature precision on the market for melting.

Rapid PCR

The RapidCycler 2 uses 1.5 mm Roche LightCycler® capillary tubes which are ideally suited for high-speed temperature cycling due to their thin walls and extremely high surface-are-to-volume ratio.

By combining glass capillary tubes and air as the cycling medium we have cut sample temperature lag to less than a second, therefore yielding high temperature ramp rates. Inside RapidCycler's cylindrical reaction chamber, tornado-like conditions ensure both temperature uniformity and rapid heat exchange within the sample. this allows the RapidCycler to spend less than one second at the high and low temperatures and still be certain that each sample has reached the target temperature.

Molecular Imaging

Xlite is a fast, easy-to-use, stand-alone imaging system dedicated to gel documentation. Based on the latest image storage technology, Xlite allows you to instantly print your gel images or save them to a memory stick via its USB port for archiving or further processing.