FilmArray...
Great things are about to happen.
The FilmArray™ is
simplified molecular testing-
easy to use, massive multiplex PCR
that turns a sample into results
in less than 1 hour.
The FilmArray–
Sample in, Results Out
-
Hi-Res Melting Instrument
-
96- or 384-well plate system
-
Results in less than 5 minutes
- Hi-Res Melting Instrument
- 32 sample carousel
- Analysis time less than 30 minutes
- Highly optimized for LCGreen Plus
Mutation Discovery
Mutations in PCR products are detected by the presence of heteroduplexes optimally detected by our LCGreen® Plus dye. Variations are identified by changes in the shape of the melting profile compared to a reference sample. Hi-Res Melting® on the LightScanner is also being used for mutation discovery in tumor samples, yielding information on mutations present in low allele fractions (as low as 5%) and allelic imbalances between tumor and matched normal samples.
Genotyping
Genotyping is possible with Hi-Res Melting eliminating the need for labeled probes. It is achieved by Hi-Res Melting® in two ways:
- With unlabeled 3' blocked probes data from the probe and PCR product can be obtained simultaneously.
- Small amplicons often as short as 50 ~ 60 bp with primers just flanking the SNP. As this application relies on subtle differences in melting temperature, it is best performed on our instruments which have the best temperature precision on the market for melting.